MicroRNA profiles in a monkey testicular injury model induced by testicular hyperthermia

نویسندگان

  • Ken Sakurai
  • Kei Mikamoto
  • Makoto Shirai
  • Takuma Iguchi
  • Kazumi Ito
  • Wataru Takasaki
  • Kazuhiko Mori
چکیده

To characterize microRNAs (miRNAs) involved in testicular toxicity in cynomolgus monkeys, miRNA profiles were investigated using next-generation sequencing (NGS), microarray and reverse transcription-quantitative real-time-PCR (RT-qPCR) methods. First, to identify organ-specific miRNAs, we compared the expression levels of miRNAs in the testes to those in representative organs (liver, heart, kidney, lung, spleen and small intestine) obtained from naïve mature male and female monkeys (n = 2/sex) using NGS analysis. Consequently, miR-34c-5p, miR-202-5p, miR-449a and miR-508-3p were identified to be testicular-specific miRNAs in cynomolgus monkeys. Next, we investigated miRNA profiles after testicular-hyperthermia (TH) treatment to determine which miRNAs are involved in testicular injury. In this experiment, mature male monkeys were divided into groups with or without TH-treatment (n = 3/group) by immersion of the testes in a water bath at 43 °C for 30 min for 5 consecutive days. As a result, TH treatment induced testicular injury in all animals, which was characterized by decreased numbers of spermatocytes and spermatids. In a microarray analysis of the testis, 11 up-regulated (>2.0 fold) and 13 down-regulated (<0.5 fold) miRNAs were detected compared with those in the control animals. Interestingly, down-regulated miRNAs included two testicular-specific miRNAs, miR-34c-5p and miR-449a, indicating their potential use as biomarkers for testicular toxicity. Furthermore, RT-qPCR analysis revealed decreased expression levels of testicular miR-34b-5p and miR-34c-5p, which are enriched in meiotic cells, reflecting the decrease in pachytene spermatocytes and spermatids after TH treatment. These results provide valuable insights into the mechanism of testicular toxicity and potential translational biomarkers for testicular toxicity. Copyright © 2016 The Authors. Journal of Applied Toxicology published by John Wiley & Sons Ltd.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Antioxidant property of Plantago major leaf extracts reduces testicular torsion/detorsion-induced ischemia/reperfusion injury in rats

The present study was aimed to determine the protective effects of Plantago major L (PM) leaf extracts on the testicular torsion/detorsion (T/D)-induced ischemia/reperfusion (I/R) injury in rats. Twenty-four mature male Sprague-Dawley rats, weighing 200-220 g, were selected. They were randomly divided into four groups of six animals each: Sham (sham-operated rats; all the surg...

متن کامل

P-3: Effects of Ethyl Pyruvate Against Methotrexate-Induced Testicular Injury

Background: Recent advances showed that oxygen radicals and hydrogen peroxides are linked with the development of several pathological processes associated with chemotherapy, including adverse effects of anti-tumor drugs. Methotrexate (MTX) is an antifolate that is widely used in the treatment of rheumatic disorders and malignant tumors. The efficacy of methotrexate is often limited by severe s...

متن کامل

P-43: Short Term Effect of Oxytocin on Testis under Ischemia Reperfusion Injury

Background: The aim of this study was to investigate the short term protective effect of oxytocin on testicular ischemia reperfusion injury immediately after detorsion in a rat model. Materials and Methods: Twenty adult rats were randomly divided into four groups: Control, Ischemia- Reperfusion (IR), Oxytocin and IR+ Oxytocin. Testicular ischemia was achieved by torsion of the left testis 720° ...

متن کامل

Radiation induced testicular injury and its amelioration by Prunus domestica in Swiss albino mice

Background: The increasing use of nuclear radiation for human welfare necessitates the search for new, safe, cost effective radio protectors not only for the personnel&rsquo;s charged with the responsibility of working or testing with radiations in laboratories, but also for general public. With this view the present study has been undertaken to determine the deleterious effects of sub lethal g...

متن کامل

Isobaric tags for relative and absolute quantification-based proteomic analysis of testis biopsies in rhesus monkeys treated with transient scrotal hyperthermia

This study aimed to examine the cellular and molecular events that occur in rhesus monkey testes after scrotal hyperthermia. Eight male adult rhesus monkeys were subjected to scrotal hyperthermia at 43°C for 30 min daily for 6 consecutive days. Sperm concentration, reproductive hormones, and testis histology were examined before hyperthermia (day 0), and at 8, 15, 30, 45, 60, 75, and 90 days af...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 36  شماره 

صفحات  -

تاریخ انتشار 2016